Home > Research groups > Wavefront-engineering microscopy (Emiliani)

Description

The investigation of signal transmission in brain requires to reproduce/observe physiological events that occur on a wide range of spatiotemporal scales, from e.g. the localized brief (μs) transient associated with asingle (sub micron) synaptic event to the complex arithmetic that dendrites use in integrating (μs-ms) multiple localizedsynaptic inputs (several micrometers apart).
Light microscopy is a fundamental tool in neuroscience offering a sensitive and non-invasive approach to probe and mimic such brain complexity.
The aim of this team is to develop a specific class of optical techniques based on spatiotemporal engineering of opticalwave-fronts obtained by phase modulation solely. This approach has the advantage of minimizing power losses, permitting a quick adaptability of the excitation pattern to the experimental context and allowing a true 3D sculpting of the excitation volumes.

The team activity is organized in four research lines:

  1. Spatiotemporal control of neuronal activity with patterned light
  2. Super resolution microscopy (STED) and scanning less microscopy
  3. Microendoscopy for awake animal imaging and for deep imaging
  4. Instrumentation



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